东海原甲藻的分子鉴定

采用nrDNAITS序列及18S序列两种分子标记,对东海原甲藻和美国国家海洋藻种保藏中心(CCMP)的具齿原甲藻进行分子鉴定,结果表明,两者之间序列非常相似,两者的nrDNAITS序列碱基差异值仅为0.002,nrDNA18S序列的碱基差异值为0.000,根据分子数据,东海原甲藻和美国国家海洋藻种保藏中心的具齿原甲藻应为同一个种.从基因库获取原甲藻的另外3个种nrD-NAITS序列和8个种的18S序列,用计算机软件进行分析和构建分子系统树,结果显示18S序列用于原甲藻的分子鉴定过于保守,而nrDNAITS更适合于该种属界定的分子标准.     

杆状病毒感染越冬亲虾(Penaeus chenesis)的研究——越冬亲虾感染及其垂直传播的可能性

在中国对虾越冬亲虾中检出杆状病毒。经对病虾鳃、前中肠、卵巢组织进行电镜观察，在这些组织中发现大量有囊膜的杆状病毒。病毒粒子大小约为３６７ｎｍ×１２８ｎｍ，核衣壳为３２４ｎｍ×９２ｎｍ。病毒在细胞内不形成包涵体，在核内或在细胞质的发生基质中装配。从病毒的形态特征、感染组织以及典型的发病症状和病理变化，初步认为亲虾感染的病毒与近年广泛报道的造成养殖对虾大规模流行病的主要原因——中国对虾杆状病毒为同一种。卵巢组织也是病毒的主要靶器官之一，亲虾卵巢组织的被感染暗示着该病毒有可能通过生殖细胞而垂直传播     

Selenium distribution in the Rhône delta and the Gulf of Lions

Total dissolved selenium and selenium species have been measured in the Rhône river delta and the Gulf of Lions within the framework of the EROS-2000 project. The Rhône river concentration of total selenium averages 2.30 nM with important variations related to river discharge. During estuar-ine mixing, the concentrations of total dissolved selenium, selenite and selenate (calculated as the difference between total dissolved and selenite) decrease linearly with increasing salinity, without significant interconversion between selenium species. In the open Mediterranean waters of the Gulf of Lions the total dissolved selenium increases from 0.5 nM in the surface waters to 0.9 nM in the deep waters. Organic selenium has been observed in Mediterranean deep water, an observation which is different from those from the Atlantic and the Pacific Oceans. The distributions of total inorganic selenium (Σ5e), selenite and selenate are strongly related to phosphate and silicate concentrations as observed previously for the major oceans.     

基于二次散射的水污染遥感模型及其在珠江口水域的应用

根据水中悬浮物、污染物和水分子等的散射和吸收物理机制建模是实现水污染定量遥感的有效途径.基于一次散射的水污染遥感模型简单易行,但在浑浊度较大时其精度不高.在基于一次散射模型的基础上分析了多次散射的能量组成特点,提出再考虑二次散射可使模型的精度显著提高且不至于使模型过于复杂;导出了二次散射的补偿因子,同时给出了考虑二次散射因子的简单计算方法.将该模型应用于珠江口水域的水污染遥感的结果表明,较之基于一次散射的模型,考虑二次散射的水污染遥感模型在精度上有明显改进.遥感提取结果显示,珠江口水域重污染区主要分布在伶仃洋东北部,香港维多利亚港和深圳湾.     

中华鲟随机扩增多态性DNA及遗传多样性研究

采用随机扩增多态性ＤＮＡ（ＲＡＰＤ）技术进行了连续３年（１９９５－１９９７）共７０尾来源于长江水系中华鲟样本遗传分析,共用了４０个１０ｂｐ长的随机引物,在２６种可供分析的引物中,只有ＯＰＫ０１、ＯＰＫ０２、ＯＰＫ０３、ＯＰＫ０９、ＯＰＫ１４和ＯＰＱ０８ＲＡＰＤ－ＰＣＲ产物有多态现象,多态引物占２３％。２６个引物中共扩增出１０８条稳定的ＤＮＡ带。其中１２条带为多态带,多态座位比例为１１．１％。个体间     

晚新生代洛川典型风尘堆积序列的延伸

对中国黄土标准剖面———洛川黄土剖面之下的上新世红黏土进行了沉积特征和磁性地层学的研究,结果表明洛川红黏土堆积于约2.6~3.2 MaBP期间,从而确定世界惟一的黄土类地质公园的风尘开始堆积的年代约为3.2 MaBP。古气候替代性指标磁化率和粒度的分析表明,洛川风尘堆积序列记录了上新世到第四纪古气候的重大转型。     

黄河三角洲潮滩剖面特征

利用验潮、潮滩水准测量、沉积物分析和遥感资料,分析了黄河三角洲不同岸段潮滩的特征。水准测量表明,侵蚀潮滩剖面形态为下凹,而淤积潮滩则上凸,由高潮线至低潮线,潮滩沉积物均变粗,这种变粗趋势在北部侵蚀岸段和河口侧缘明显,在河口南侧稳定海岸则不明显。潮滩沉积物含水量为12.2%~32.2%,平均为21%,海滩表层沉积物不排水剪切强度在0.1~0.35 kg/cm2之间。受局限岸滩的平面形态和剖面形态受到周围大坝地形影响,具有和砂质海滩类似的形态,显示波浪对该潮滩的作用强烈,根据剖面测量结果与遥感图像,发现河口北侧有一个小的冲积扇。     

In-Situ Observation of Fluid Mud in the North Passage of Yangtze Estuary, China

Observations of fluid mud were made in the lower North Passage of the Yangtze Estuary in February 2000, on 10 -11 August 2000, on 30 - 31 August 2000 (after two strong typhoons), on 21 - 24 August 2000 (neap tide) and on 3 -6 September 2000 (mean tide) respectively. In situ data show that the fluid mud in this area consists of fine cohesive sediment (median size 7.23 μm). The formation and movement of fluid mud varied during the neap-spring and flood-ebb tidal cycle. Observations suggest that fluid mud phenomena in this area may be categorised in a three-fold manner as slack water, storm and saltwedge features. The thickness of the fluid mud layer of slack water during the neap tide ranged from 0.2 to 0.96 m, whereas during the mean tide, the thickness ranged from 0.17 to 0.73 m, and the thickness of the fluid mud layer was larger during slack water than at the flood peak. Shoals cover an area of 800 km^2 with a water depth smaller than 5 m. Erosion of these extensive intertidal mudflats due to storm action provides an abundant sediment source. This is particularly significant in this estuary when the tidal level is lower than 5 m. The lower North Passage is a typical zone of saltwater wedging, so the saltwedge fluid mud has the most extensive spatial range in the estuary.     

光生物反应器中光衰减特征与螺旋藻生长动力学研究

分析了光照强度在光生物反应器中的分布特征，结果表明，当光波长及光传播的路径确定时，光生物反应器中光衰减特征主要受培养物生物量浓度的影响，由回归的模型对实验数据的拟合可分析光衰减特征与培养物生物量浓度的相关性，为光生物反应器中平均光照强度的确定奠定基础。在光生物反应器中，当营养底物和环境温度不是螺旋藻生长限制因子时，通过平均光照强度对螺旋藻比生长速率的影响分析，结果表明，在实验条件下，螺旋藻比生长速率与平均光照强度的动力学模型可用Aiba光生长抑制方程描述，光亲和系数Ks为238.29umol/(m^2.s)，光抑制系数Ki为0．00493s.m^2/umol，光生物反应器中螺旋藻生长的饱和光照强度出现在190－272umol/(m^2.s)的范围内。